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Melao-motheo le Mekhoa ea Tlhahlobo ea Bongata ka Liquid Chromatography

Melao-motheo le Mekhoa ea Tlhahlobo ea Bongata ka Liquid Chromatography

 

Mokhoa oa karohano oa chromatography ea mokelikeli o ipapisitse le phapang ea kamano ea likarolo tsa motsoako bakeng sa mekhahlelo e 'meli.

Ho ea ka mekhahlelo e fapaneng ea ho ema, chromatography ea mokelikeli e arotsoe ka chromatography e tiileng ea metsi, chromatography ea mokelikeli le chromatography ea bonded phase.Tse sebelisoang haholo ke mokelikeli-solid chromatography e nang le silica gel e le filler le bonded phase chromatography e nang le microsilica joalo ka matrix.

Ho ea ka mofuta oa mokhahlelo o emeng, chromatography ea metsi e ka aroloa ka kholomo ea chromatography, chromatography ea pampiri le chromatography e tšesaane.Ho ea ka matla a adsorption, e ka aroloa ka chromatography ea adsorption, partition chromatography, ion exchange chromatography le gel permeation chromatography.

Lilemong tsa morao tjena, tsamaiso e phahameng ea khatello ea metsi e kenyelelitsoe tsamaisong ea chromatography ea mokelikeli ho etsa hore mohato oa selefouno o phalle ka potlako tlas'a khatello e phahameng ea ho ntlafatsa phello ea karohano, kahoo e sebetsa hantle haholo (e tsejoang hape e le "high-pressure) liquid chromatography. e hlahile.

KAROLO
01 Molao-motheo oa Tlhahlobo ea Bongata ba Mokelikeli oa Chromatography

Ho lekanya motheong oa boleng, lintho tse hloekileng lia hlokahala e le litekanyetso;

Liquid chromatography quantification ke mokhoa o batlang o le ngata: ke hore, palo ea analyte motsoakong e hakanngoa ho tsoa ho palo e tsebahalang ea sampole e hloekileng e tloaelehileng.

KAROLO
02 Motheo oa Quantification ka Liquid Chromatography

Palo ea karolo e lekantsoeng (W) e lekana le boleng ba karabo (A) (bophahamo ba tlhōrō kapa sebaka se phahameng), W=f×A.

Quantitative correction factor (f): Ke proportionality constant of the quantitative calculation formula, and its physical meaning ke palo ya karolo e lekantsweng e emetsweng ke yuniti ya karabelo ya boleng (sehlohlolong).

Ntho ea tokiso ea bongata e ka fumanoa ho palo e tsejoang ea sampole e tloaelehileng le boleng ba eona ba karabo.

Lekanya boleng ba karabo ea karolo e sa tsejoeng, 'me palo ea karolo e ka fumanoa ka tekanyo ea ho lokisa palo.

KAROLO
03 Mantsoe a tloaelehileng tlhahlobong ea bongata

Mohlala (sample): tharollo e nang le analyte bakeng sa tlhahlobo ea chromatographic.E arotsoe ka mehlala e tloaelehileng le e sa tsejoeng.

Standard: Sehlahisoa se hloekileng se nang le mohopolo o tsebahalang.Sampole e sa tsejoeng (ha e tsejoe): Motsoako oo mahloriso a oona a lokelang ho lekoa.

Boima ba mohlala: Boima ba pele ba sampole e lokelang ho lekoa.

Dilution: Ntho ya dilution ya sampole e sa tsejweng.

Karolo : tlhoro ea chromatographic e lokelang ho hlahlojoa ka bongata, ke hore, mohlahlobi eo litaba tsa hae li sa tsejoeng.

Palo ea karolo (chelete): likahare (kapa mahloriso) a ntho e lokelang ho lekoa.

Botšepehi : Mokhoa oa khomphutha oa ho lekanya sebaka se phahameng sa tlhoro ea chromatographic ka komporo.

Calibration Curve: Mokokotlo o motala oa dikahare ho bapisa boleng ba karabelo, o thehilwe ho tswa ho palo e tsebahalang ya ntho e tlwaelehileng, e sebedisetswang ho tseba hore na ke eng e sa tsejweng ya mohlahlobi.

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KAROLO
04 Tlhahlobo ea Palo ea Liquid Chromatography

1. Khetha mokhoa oa chromatographic o loketseng tlhahlobo ea palo:

l Netefatsa tlhoro ea karolo e fumanoeng 'me u fihlele qeto (R) e kholo ho feta 1.5

l Etsa qeto ea ho lumellana (ho hloeka) ha litlhōrō tsa chromatographic tsa likarolo tse lekiloeng

l Etsa qeto ea moeli oa ho lemoha le moeli oa palo ea mokhoa;sensitivity le linear range

2. Theha sekhahla sa calibration ka lisampole tse tloaelehileng tsa likhakanyo tse fapaneng

3. Hlahloba ho nepahala le ho nepahala ha mekhoa ea palo

4. Sebelisa software e tsamaellanang ea tsamaiso ea chromatography ho kenya ts'ebetsong pokello ea mehlala, ts'ebetso ea data le liphetho tsa tlaleho

KAROLO
05 Ho tsebahatsa litlhoro tsa bongata (boleng)

Hlalosa ka nepo tlhoro e 'ngoe le e 'ngoe ea chromatographic e lokelang ho baloa

Taba ea pele, sebelisa sampole e tloaelehileng ho fumana nako ea ho boloka (Rt) ea tlhoro ea chromatographic e lokelang ho baloa.Ka ho bapisa nako ea ho boloka, fumana karolo e tsamaellanang le tlhoro e 'ngoe le e 'ngoe ea chromatographic sampole e sa tsejoeng.Mokhoa oa boleng ba chromatographic ke ho bapisa nako ea ho boloka le sampole e tloaelehileng.Maemo a sa lekananetefatso e eketsehileng (boleng)

1. Mokhoa o tloaelehileng oa tlatsetso

2. Sebelisa mekhoa e meng ka nako e le 'ngoe: mekhoa e meng ea chromatographic (fetola mochine, joalo ka: ho sebelisa likholomo tse fapaneng tsa chromatographic), li-detectors tse ling (PDA: papiso ea spectrum, patlisiso ea laebrari ea spectrum; MS: tlhahlobo ea spectrum spectrum, spectrum library search)

3. Lisebelisoa tse ling le mekhoa

KAROLO
06 Netefatso ea Quantitative Peak Consistency

Netefatsa ho lumellana ha tlhoro ea chromatographic (bohloeki)

Netefatsa hore ho na le karolo e le 'ngoe feela e lekantsoeng tlas'a tlhoro e 'ngoe le e 'ngoe ea chromatographic

Sheba hore na ha ho na tšitiso e tsoang ho co-eluting substances (litšila)

Mekhoa ea Netefatso ea Chromatographic Peak Consistency (Bohloeki)

Ho bapisa li-Spectrograms le li-Detector tsa Photodiode Matrix (PDA).

Peak Purity Identification

2996 Purity Angle Theory

Mekhoa e mengata e sebelisoang hangata KAROLO EA 07

Mokhoa o tloaelehileng oa curve, o arotsoe ka mokhoa o tloaelehileng oa kantle le mokhoa o tloaelehileng oa kahare:

1. Mokhoa o tloaelehileng oa ka ntle: e sebelisoang haholo ho chromatography ea metsi

Letoto la lisampole tse tloaelehileng tsa likhakanyo tse tsebahalang li ile tsa lokisoa ho sebelisoa lisampole tse hloekileng tsa metsoako e lokelang ho lekoa joalo ka lisampole tse tloaelehileng.e kentsoe kholomong ho fihlela boleng ba eona ba karabelo (sebaka sa tlhoro).
Ka har'a mefuta e itseng, ho na le kamano e ntle ea mola pakeng tsa khokahanyo ea sampole e tloaelehileng le boleng ba karabelo, e leng W= f×A , 'me ho etsoa lekhalo le tloaelehileng.

Tlas'a maemo a ts'oanang a liteko, kenya sampole e sa tsejoeng ho fumana boleng ba karabo ea karolo e lokelang ho lekanyetsoa .Ho ea ka coefficient f e tsejoang, ho ka fumanoa motsoako oa karolo e lokelang ho lekanyetsoa.

Melemo ea mokhoa o tloaelehileng oa kantle:ts'ebetso e bonolo le ho bala, ke mokhoa o sebelisoang hangata;ha ho na tlhoko ea hore karolo e 'ngoe le e' ngoe e bonwe le ho hlakoloa;ho hlokahala sampole e tloaelehileng;maemo a ho lekanya a sampole e tloaelehileng le sampole e sa tsejoeng e lokela ho lumellana;molumo oa ente e lokela ho ba o nepahetseng.

Mefokolo ea mokhoa o tloaelehileng oa kantle:Maemo a liteko a hlokeha hore a be a phahameng, joalo ka kutloisiso ea detector, lebelo la phallo, le ho hlophisoa ha mohato oa mohala o ke keng oa fetoloa;bophahamo ba ente ka 'ngoe e lokela ho ba le pheta-pheta e ntle.

2. Mokhoa o tloaelehileng oa ka hare: e nepahetse, empa e le mathata, e sebelisoang haholo mekhoeng e tloaelehileng

Palo e tsejoang ea tekanyo ea ka hare e kenyelletsoa ho tekanyo ea ho etsa maemo a tsoakiloeng, 'me letoto la litekanyetso tsa ho sebetsa tsa mahloriso a tsejoang li lokiselitsoe.Karolelano ea molar ea maemo a tloaelehileng ho ea ka hare ho litekanyetso tse tsoakiloeng e lula e sa fetohe.Entela kholomong ea chromatographic 'me u nke (sehlohlolo se tloaelehileng sa sampole/sebaka sa tlhōrō se tloaelehileng sa ka hare) e le boleng ba karabo.Ho ea ka kamano ea mola pakeng tsa boleng ba karabo le kholiso ea maemo a sebetsang, e leng W= f×A, ho etsoa lekhalo le tloaelehileng.

Palo e tsejoang ea maemo a ka hare e eketsoa ho sampole e sa tsejoeng ebe e kenngoa kholomong ho fumana boleng ba karabo ea karolo e lekantsoeng .Ho ea ka coefficient f e tsejoang, ho ka fumanoa motsoako oa karolo e lokelang ho lekanyetsoa.

Litšobotsi tsa mokhoa o tloaelehileng oa kahare:Nakong ea ts'ebetso, sampuli le maemo a ka hare li kopantsoe hammoho 'me li kenngoa ka har'a kholomo ea chromatographic, hafeela karo-karolelano ea karolo e lekantsoeng ho ea ka hare ho tharollo e tsoakiloeng e sa fetohe, phetoho ea molumo oa sampole. e ke ke ea ama liphetho tsa bongata..Mokhoa o tloaelehileng oa ka hare o fokotsa tšusumetso ea molumo oa sampuli, esita le mohato oa selefouno le detector, kahoo e nepahetse ho feta mokhoa o tloaelehileng oa kantle.

1668066397707 SAEWBVKAROLO
Lintlha tse 08 tse Amang Liphetho tsa Tlhahlobo ea Bongata

Ho se nepahale hantle ho ka bakoa ke:

Khokahano e fosahetseng ea sebaka sa tlhoro, ho bola ha sampole kapa litšila tse hlahisitsoeng nakong ea ho lokisoa, sampole ea vial e sa koaloang, sampole kapa solvent volatilization, tokiso e fosahetseng ea sampole, mathata a ente ea sampole, boitokisetso bo fosahetseng ba ka hare.

Mabaka a ka bakang ho se nepahale hantle:

Khokahano e fosahetseng ea tlhoro, mathata a ente kapa ente, ho bola ha sampole kapa litšila tse hlahisitsoeng nakong ea tokiso ea sampole, mathata a chromatographic, karabelo e senyehileng ea sesebelisoa.

 


Nako ea poso: Nov-10-2022