Ka kopo iketsetse khetho ea hau ho latela maemo a laboratori ea hau.
Ho bohlokoa ho fumana mokhoa o sebetsang oa ho hloekisa li-vials tsa mohlala
Hona joale, ho na le palo e kholo ea lihlahisoa tsa temo (lihlahisoa tse ling tsa lik'hemik'hale, li-organic acid, joalo-joalo) tse lokelang ho lekoa ka chromatography ea metsi le chromatography ea khase selemo se seng le se seng.Ka lebaka la palo e kholo ea lisampole, ho na le palo e kholo ea linkho tsa mohlala tse hlokang ho hloekisoa nakong ea ts'ebetso ea ho sibolla, e sa senyeng nako feela le ho fokotsa ts'ebetso ea mosebetsi, empa hape ka linako tse ling e baka ho kheloha liphethong tsa liteko ka lebaka la bohloeki. linkho tsa sampole tse hloekisitsoeng.
Likotlolo tsa mohlala oa chromatographic li entsoe haholo ka khalase, hangata li etsoa ka polasetiki.Linkho tsa mohlala tse lahliloeng li turu, lia senya, ebile li silafatsa tikoloho.Lilaboratori tse ngata li hloekisa linkho tsa sampole ebe li li sebelisa hape.
Hajoale, mekhoa e sebelisoang hangata ea laboratori ea ho hlatsoa linkho ke haholo-holo e kentsoeng sesepa, sesepa, lihlapolli tsa manyolo le ho hlatsoa asiti, ebe ho etsoa borashe tsamaiso e nyane ea li-tubes.
Mokhoa ona o tloaelehileng oa ho hohla o na le mefokolo e mengata:
Tšebeliso ea sesepa e sebelisa metsi haholo, nako ea ho hlatsoa e telele, 'me ho na le likhutlo tse thata ho hloekisa.Haeba e le linkho tsa mohlala tsa polasetiki, ho bonolo ho ba le matšoao a borashe ka har'a lebota la linkho, tse nkang lisebelisoa tse ngata tsa basebetsi.Bakeng sa lisebelisoa tsa khalase tse silafalitsoeng haholo ke masala a lipid le protheine, tharollo ea alkaline lysis e sebelisetsoa ho hloekisa, 'me liphello tse ntle li finyelloa.
Ha ho hlahlojoa disampole ka LC/MS/MS, ho hloekisoa ha linkho tsa ente ho bohlokoa haholo.Ho ea ka mokhoa oa ho hloekisa oa glassware, mokhoa oa ho hloekisa o khethoa ho ea ka tekanyo ea tšilafalo.Ha ho na mokhoa o tsitsitseng.Kakaretso ea mokhoa:
Khetho ea Pele:
1. Tšela tharollo ea teko ka har'a linkho tse omeletseng
2. Kenya tharollo eohle ea teko ka 95% ea joala, e hlatsoe ka makhetlo a mabeli ka ultrasonic 'me ue tšollele, hobane joala bo kena habonolo ka har'a vial ea 1.5mL 'me e ka ba miscible le li-solvents tse ngata ho finyella phello ea ho hloekisa.
3. Tšela ka metsi a hloekileng, 'me u hlatsoe ka ultrasonically ka makhetlo a mabeli.
4. Tšela lotion ka har'a linkho tse omeletseng ebe u chesa ka likhato tse 110 tsa Celsius bakeng sa lihora tse 1 ho isa ho tse peli.Le ka mohla u se ke ua baka mocheso o phahameng.
5. Pholisa 'me u boloke.
Khetho ea bobeli:
1. Hlatsoa ka metsi a pompo ka makhetlo a 'maloa
2. E kenye ka beaker e tletseng metsi a hloekileng (Millipore pure water machine) 'me u sonicate ka metsotso e 15.
3. Fetola metsi le ultrasound metsotso e 15
4. Inela ka bekeng e tlatsitsoeng ka ethanol e felletseng (Sinopharm Group, Analytical Pure)
5. Qetellong, e ntše 'me u e tlohele hore e omelle.
Khetho ea boraro:
1. Pele u kene ka methanol (chromatographically pure), 'me u hloekise ka ultrasonically ka metsotso e 20, ebe u tšela methanol e omme.
2. Tlatsa linkho tsa mohlala ka metsi, 'me u hloekise ka ultrasonically metsotso ea 20, tšela metsi .
3. Omisa linkho tsa mohlala ka mor'a moo.
Khetho ea Bone:
Mokhoa oa ho hlatsoa oa li-vials tsa mohlala o tšoana le ho lokisetsa karolo ea mokelikeli, joalo-joalo, pele, sebelisa joala ea bongaka ho soak ka lihora tse fetang 4, ebe ultrasound bakeng sa halofo ea hora, ebe u tšela joala ea bongaka, 'me u sebelise metsi. bakeng sa halofo ea ultrasound.Lihora, hlatsoa ka metsi 'me ue omise.
Khetho ea bohlano:
Ntlha ea pele, inela ka tharollo e matla ea ho hloekisa oxidizing (potassium dichromate) bakeng sa lihora tse 24, ebe u sebelisa metsi a deionized ka ultrasonic Hlatsoa ka makhetlo a mararo tlas'a maemo, 'me qetellong ue hlatsoe ka methanol hanngoe, ebe u e omisa hore e sebelisoe.
Li-caps septas li tlameha ho nkeloa sebaka, haholo-holo ha ho hlahlojoa masalla a chefo e bolaeang likokoanyana, ho seng joalo e tla ama liphello tsa palo.
Empa haeba maemo a lumela, leka ho sebelisa lisebelisoa tse ka lahloang, joalo ka lisebelisoa tsa PTFE tse ka lahloang kapa tse kenyang polasetiki ea lapeng (hoo e ka bang 0.1 yuan/piece), le linkho tsa sampole li lokile.Tšebeliso e pheta-phetoang 'me ha e hloke ho hloekisoa.
Khetho ea botšelela:
(1) mokhoa o rarahaneng oa ho hloekisa o nang le liphetho tse sebetsang:
No1.Ka mor'a hore ho sebelisoe linkho tsa mohlala, hlatsoa linkho tsa mohlala ka metsi a phallang pele, 'me u hlatsoe sampuli e setseng (o ka e sisinya ka letsoho ka nako e le' ngoe);
No2, ebe u kenya linkho tsa sampuli ka har'a bubble ea metsi a hlatsoang dichromate ea potassium, 'me ha e bokella Ha u fihla tekanyo e itseng kapa ha u le boemong bo botle, e ntše ka har'a tanka ea lotion' me ue kenye ka har'a sefe ea polasetiki bakeng sa kichineng. tshebediso.Hlatsoa hantle ka metsi a pompo.U ka sefa khafetsa le ho sisinya bohareng;
No3.Sebelisa metsi a pompo ho hloekisa ka ultrasonically makhetlo a 3 ka mor'a ho hlatsoa.Ho pota-pota, ho molemo ho sisinya metsi ka har'a lijana tsa mohlala ka mor'a ho hloekisa ka 'ngoe ea ultrasonic;
No4, joale sebelisa metsi a mararo a silafetseng (kapa metsi a hloekisitsoeng, metsi a hloekisitsoeng) ka 1.3 ultrasonic hloekisa ka makhetlo a mararo;
No5, ebe sebelisa chromatographic pure methanol ultrasonic hloekisa makhetlo a 2-3, ho boetse ho molemo ho
sisinya methanol ka har'a linkho tsa sampole ka mor'a ho hloekisa;
No6.Beha linkho tsa mohlala ka ontong 'me u li omise ka likhato tse ka bang 80,' me li ka sebelisoa.
(2) linkho tsa mohlala tse rekiloeng ho tšoaea ka mebala e fapaneng:
haeba u hlokometse hore ho na le letšoao le lenyenyane la 'mala holim'a lijana tsa mohlala, e seng bakeng sa ponahalo e ntle, empa e na le tšebeliso ea eona.Ha u reka, ho molemo ho reka li-vial tse 'maloa tsa mebala e sa tšoaneng.
Mohlala: laboratori ea hau e bula liporojeke tse peli A le B ka nako e le 'ngoe.Lekhetlo la pele A projeke e sebelisa linkho tse tšoeu tsa sampole, mme morero oa B o sebelisa libotlolo tsa sampole tse putsoa.Ka mor'a hore tlhahlobo e phethoe, e hloekisoa ho ea ka mokhoa o ka holimo, 'me teko ea bobeli Ka nako eo, sebelisa li-vial tsa mohlala tse putsoa bakeng sa morero oa A, lisebelisoa tse tšoeu tsa sampole bakeng sa morero oa B, joalo-joalo, tse ka qobang mathata a bakoang ke ka katleho. tshilafatso mosebetsing wa hao.
Ngola qetellong
1. Lienjiniere tse 'maloa tsa lisebelisoa li khothalelitse: Sebelisa sebōpi sa muffle ka likhato tse 400 ho baka halofo ea hora, lintho tse phelang li felile;
2. Kenya linkho tsa mohlala ka seboping sa muffle bakeng sa ho omisoa ka likhato tse 300 tsa Celsius.Moenjiniere oa Agilent oa Beijing o itse ha a fihla seboping sa muffle, tlhahlobo e ke ke ea e-ba lerata ka mor'a ho baka ka sebōping sa muffle ka likhato tse 300 ka lihora tse 6.
hape ………..
Li-flasks tse nyenyane tsa volumetric, li-flasks tse bōpehileng joaloka pere bakeng sa mouoane oa rotary, le lisebelisoa tse ling tsa khalase bakeng sa ho hlahlojoa kapa ho hlahlojoa esale pele li ka hloekisoa ka ho bua ka mokhoa ona.
Nako ea poso: Feb-25-2022